NF-κBp65在创伤性关节炎中的表达

陈连旭 于长隆 王海军 林霖 魏学磊 傅欣 张继英

北京大学第三医院运动医学研究所 (北京 100083 )

摘要 目的 观察实验性创伤性关节炎的软骨和滑膜组织中NF-κBp65的表达情况。 材料和方法 取雄性S-D大鼠作为实验动物，切断膝关节的内侧副韧带和部分切除内侧半月板制作创伤性关节炎模型。蛋白质免疫印迹法（Western Blot）和免疫组织化学染色检测关节软骨和滑膜内的NF-κBp65表达情况。 结果 与正常关节软骨和滑膜相比，创伤性关节炎的软骨和滑膜中的NF-κBp65表达明显增高。结论 NF-κBp65可能在创伤性关节炎的发生过程中发挥作用

[1]【关键词】 创伤性关节炎；NF-κBp65；Western Blot；免疫组化染色

The expression of NF-κBp65 in traumatic osteoarthritis

Chen lianxu, Yu changlong, Wang haijun, Lin lin, Wei xuelei, Fu xin, Zhzng jiying

Institute of Sports Medicine, Peking University Third hospital, Beijing,China, 100083

Abstract Objective To observe the expression of NF-κBp65 in cartilage and synovium of experimental traumatic osteoarthritis. Materials and Methods The animal models of osteoarthritis were male S-D rats which transection of the medial collateral ligament plus partial medial meniscectomy. Western Blot and immunohistochemical staining were made to detect the expression of NF-κBp65 in cartilage and synovium. Results The expression of NF-κBp65 in cartilage and synovium of tranmatic osteoarthritis was more increased than of normal joint. Conclusion NF-κBp65 may play important role in initiation and development of traumatic osteoarthritis.

Key words traumatic osteoarthritis, NF-κBp65, Western Blot, immunohistochemical staining